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CRK12 and CYC9 interact in a yeast two hybrid assay. A: β-galactosidase assay for transcription of LacZAs well as in vivo antileishmanial efficacy of a mixture therapy of diminazene and artesunate in opposition to Leishmania donovaniTo make sure the right orientation with the inserted fragments while in the CRK12-RNAi assemble, PCR and sequencing